en بیوشیمی Biochemistry Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> Tumor necrosis factor alpha (TNF&alpha;) is a 17 kDa, an important soluble pro-inflammatory cytokine, which is involved in some tissue dysfunctions, including thyroid and liver tissue. In spite of its role in thyroid and tissue damage separately, the relationship between this factor and these two disorders has not been clarified. The aim of the present study was to evaluate liver biochemical parameters and TNF&alpha; in hypothyroid patients compared to euthyroid subjects.<br> <b>Methods:</b> To achieve this purpose, samples were transferred into tubes without anticoagulants and then centrifuged immediately to separate the serum. All markers in the serum were measured using commercial kits, including T3, T4, TSH, and TNF&alpha;, which were detected using the ELISA method. Liver function tests, including albumin, total bilirubin, and total protein were measured by spectroscopy and the colorimetric method, respectively. In addition, AST, ALT, ALP, and GGT were detected using enzymatic methods.<br> <b>Results:</b> Our results showed that the level of TNF&alpha; in hypothyroid patients was significantly higher than that in normal individuals (P = 0.009). TNF&alpha; had a significantly positive correlation with TSH and T3 but a negative correlation with T4. Furthermore, AST, ALT, and GGT had a positive correlation with TSH and a negative correlation with albumin, total protein, and total bilirubin. These correlations were insignificant (P < 0.05).<br> <b>Conclusion</b>: According to our data, the positive correlation of TSH with both TNF&alpha; and liver function tests may indicate a relationship between thyroid and liver function with each other.</span></span></div> Hypothyroidism, Tumor necrosis factor-alpha, Liver function tests, Gamma-Glutamyltransferase, Total protein 31 33 http://mlj.goums.ac.ir/browse.php?a_code=A-10-682-1&slc_lang=en&sid=1 Noori Noha Alsharifi noorinoah85@gmail.com 100319475328460029236 0009-0007-7790-3522 No Department of Clinical Biochemistry, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran Mahin Gholipur dr_gholipour@yahoo.com 100319475328460029237 100319475328460029237 No Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran Somayeh Ghorbani s.ghorbani.gh@gmail.com 100319475328460029238 100319475328460029238 No Cancer Research Center, Golestan University of Medical Sciences, Gorgan, Iran Fatemeh Mohammadzadeh msoheila66@yahoo.com 100319475328460029239 100319475328460029239 No Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran Safoura Khajeniazi niazie80@gmail.com 100319475328460029240 100319475328460029240 Yes Department of Clinical Biochemistry, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran ; Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran