en پزشکی مولکولی Molecular Medicine Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> A rare heterozygous DYRK1B mutation (R102C) recently linked to a familial form of metabolic syndrome prompted this study to introduce the R102C mutation into the mouse <i>DYRK1B gene</i>, utilizing recombinant lentiviruses for long-term gene expression.<b><span style="font-size:8.0pt"></span></b><br> <b>Methods:</b> In the present fundamental study, the DYRK1B R102C mutation was generated via Overlap Extension-PCR (OE-PCR) and inserted into the LeGO-iG2 transfer vector with a GFP marker. Recombinant lentiviruses were produced by co-transfection of the transfer vector carrying DYRK1B R102C, psPAX2 (Packaging vector), and pMD2 (Envelope vector) into HEK-293T cells.<br> <b>Results:</b> The accuracy of the intended mutation was confirmed through OE-PCR and sequencing. Expression of DYRK1B and successful gene transfer were visualized using a fluorescence microscope to detect the GFP marker. Lentiviral titer was quantified using flow cytometry, with an infection efficiency of 108 TU/ml in HEK-293T cells.<br> <b>Conclusion</b>: DYRK1B plays a crucial role in the pathogenesis of metabolic syndrome, central obesity, early-onset coronary artery disease, hypertension, type 2 diabetes, and adipogenesis, suggesting its potential as a target for therapeutic interventions. Lentiviruses carrying the DYRK1B R102C mutation offer significant advantages for both <i>in vitro</i> and <i>in vivo</i> research on metabolic syndrome. This study showcases the successful application of recombinant lentiviral vectors for gene transfer into eukaryotic cells.</span></span><br> &nbsp;</div> DYRK1B protein, human, Metabolic syndrome, Mutagenesis, Cloning, Organism, Lentivirus, Genetic vectors 34 38 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1634-1&slc_lang=en&sid=1 Afrooz Daneshparvar afrooz.daneshparvar2017@gmail.com 100319475328460029241 100319475328460029241 No Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran ; Molecular Dermatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Iman Jamhiri i.jamhiri@gmail.com 100319475328460029242 100319475328460029242 No Molecular Dermatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Vahid Razban razban_vahid@yahoo.com 100319475328460029243 100319475328460029243 No Department of Molecular Medicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran Jafar Fallahi jafarf80@gmail.com 100319475328460029244 100319475328460029244 No Department of Molecular Medicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran Nasrin Hamidizadeh n.hamidizadeh.mdrc@gmail.com 100319475328460029245 100319475328460029245 No Molecular Dermatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Behnam Moghtaderi behnam_moghtaderi@yahoo.com 100319475328460029246 0009-0007-7789-8639 No Department of Pathobiology, Shiraz Veterinary Medicine, Shiraz University, Shiraz, Iran Mehdi Dianatpour mdianatpur@gmail.com 100319475328460029247 100319475328460029247 Yes Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Human Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran