Medical Laboratory Journal

en Early Detection of Antibiotic Resistance in Positive Blood Cultures: A Study from a Tertiary Care Center in India باکتری شناسی bacteriology تحقيقي Original Paper <div style="text-align: justify;">Background: Conventional culture and sensitivity methods take around 48 hours to generate antibiotic sensitivity results after a blood culture is flagged as positive by automated systems. However, it is imperative to initiate early targeted antibiotic therapy for effective management of sepsis and to reduce morbidity, mortality, and cost of treatment. This study aimed to evaluate the direct sensitivity test (DST) as a potential tool to obtain quicker antibiotic susceptibility results from positive BacT/ALERT blood culture vials and the VITEK-2 system (the reference method). Methods: Blood culture bottles flagged as positive by BacT/ALERT were Gram-stained. Cultures with polymicrobial growth were excluded from the study. The isolates were then simultaneously cultured and processed for the DST using the disk diffusion method. Agreements or errors were interpreted according to the Clinical and Laboratory Standards Institute’s guidelines. Results: Among 76 Gram-positive isolates, we observed 99.2% essential agreement between the DST and AST. The rate of minor and major errors was 4.04% and 1.18%, respectively. Among 75 Gram-negative isolates, we observed 98.99% essential agreement between the DST and AST. The rate of minor and major errors was 4% and 2%, respectively. No very major error was seen in either Gram-negative or -positive isolates. Conclusions: The DST results are available earlier than the AST results, which can ultimately help in the early initiation of targeted antibiotic therapy. </div> Drug Resistance, Microbial,blood culture,Sepsis, 8 14 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1254-1&slc_lang=en&sid=1 Sadaf Khursheed Baba Sadafbaba7@gmail.com 100319475328460031989 100319475328460031989 No Department of Microbiology, Sheri-Kashmir Institute of Medical Sciences, Srinagar, India Abiroo Jan abroo_naqash@yahoo.com 100319475328460031990 100319475328460031990 Yes Department of Microbiology, Government Medical College, Anantnag, India Mohd Suhail lone drmohmmadsuhail@gmail.com 100319475328460031991 100319475328460031991 No Pediatric Microbiology GMC Srinagar, India Dalip k kakru dkkakru@yahoo.co.in 100319475328460031992 100319475328460031992 No Microbiology SMSR, Sharda University Knowledge Park 3, Greater Noida, India Bashir Ahmad Fomda bashirfomda@gmail.com 100319475328460031993 100319475328460031993 No Department of Microbiology Sher-i-Kashmir Institute of Medical Sciences, Srinagar, India Gulnaz Bashir drgulnazbashir@hotmail.com 100319475328460031994 100319475328460031994 No Department of Microbiology Sher-i-Kashmir Institute of Medical Sciences, Srinagar, India Nadeem ahmad bhat Nadeembhat.092@gmail.com 100319475328460031995 100319475328460031995 No Department of Microbiology, Sheri-Kashmir Institute of Medical Sciences, Srinagar, India